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Merck

PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus.

New biotechnology (2010-04-21)
Glenn E Bartley, Andrew P Breksa, Betty K Ishida
RESUMEN

The phenolic amine synephrine is a vascoconstrictor and bronchiectatic agent and holds promise as an aid to weight management and obesity reduction. Synephrine is structurally similar to the active ingredients of several commercial cold remedies. Some Citrus contain high concentrations of synephrine. An enzyme involved in synephrine biosynthesis, tyrosine decarboxylase (TYDC), is a pyridoxal-5'-phosphate (PLP)-dependent enzyme that decarboxylates tyrosine to yield CO(2) and tyramine. We used PCR to screen, clone and sequence this gene from various synephrine producing and nonproducing Citrus species and varieties to determine if DNA sequence of this gene correlated with synephrine presence. PCR amplification and comparison of DNA sequence indicates DNA sequence differences that may cause production of truncated proteins to be produced in some nonsynephrine producing Citrus. Synephrine production may be genetically determined in part by the gene for TYDC.

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Sigma-Aldrich
L-Tyrosine Decarboxylase Apoenzyme from Streptococcus faecalis, <0.005 unit/mg solid (without pyridoxal 5-phosphate), ≥0.05 unit/mg solid (with excess pyridoxal 5-phosphate)
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Número de referencia del producto (SKU)Tamaño de envaseDisponibilidadPrecio
200 μg
Fecha estimada de envío 23 de marzo de 2025
Está disponible un anticuerpo recombinante y sin conservantes para su diana. Pruebe ZRB1226
USD 660.00

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Sigma-Aldrich
L-Tyrosine Decarboxylase from Streptococcus faecalis, ≥0.1 unit/mg solid
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Número de referencia del producto (SKU)Tamaño de envaseDisponibilidadPrecio
200 μg
Fecha estimada de envío 23 de marzo de 2025
Está disponible un anticuerpo recombinante y sin conservantes para su diana. Pruebe ZRB1226
USD 660.00

Para encargar productos, Contacto su distribuidor local.