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CD66b-CD64dimCD115- cells in the human bone marrow represent neutrophil-committed progenitors.

Nature immunology (2022-04-29)
Federica Calzetti, Giulia Finotti, Nicola Tamassia, Francisco Bianchetto-Aguilera, Monica Castellucci, Stefania Canè, Silvia Lonardi, Chiara Cavallini, Alessandro Matte, Sara Gasperini, Ilaria Signoretto, Fabio Benedetti, Massimiliano Bonifacio, William Vermi, Stefano Ugel, Vincenzo Bronte, Cristina Tecchio, Patrizia Scapini, Marco A Cassatella
RESUMEN

Here we report the identification of human CD66b-CD64dimCD115- neutrophil-committed progenitor cells (NCPs) within the SSCloCD45dimCD34+ and CD34dim/- subsets in the bone marrow. NCPs were either CD45RA+ or CD45RA-, and in vitro experiments showed that CD45RA acquisition was not mandatory for their maturation process. NCPs exclusively generated human CD66b+ neutrophils in both in vitro differentiation and in vivo adoptive transfer experiments. Single-cell RNA-sequencing analysis indicated NCPs fell into four clusters, characterized by different maturation stages and distributed along two differentiation routes. One of the clusters was characterized by an interferon-stimulated gene signature, consistent with the reported expansion of peripheral mature neutrophil subsets that express interferon-stimulated genes in diseased individuals. Finally, comparison of transcriptomic and phenotypic profiles indicated NCPs represented earlier neutrophil precursors than the previously described early neutrophil progenitors (eNePs), proNeus and COVID-19 proNeus. Altogether, our data shed light on the very early phases of neutrophil ontogeny.

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Anti-LTF antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution