Saltar al contenido
Merck
  • The Translational Machinery of Human CD4+ T Cells Is Poised for Activation and Controls the Switch from Quiescence to Metabolic Remodeling.

The Translational Machinery of Human CD4+ T Cells Is Poised for Activation and Controls the Switch from Quiescence to Metabolic Remodeling.

Cell metabolism (2018-09-11)
Sara Ricciardi, Nicola Manfrini, Roberta Alfieri, Piera Calamita, Maria Cristina Crosti, Simone Gallo, Rolf Müller, Massimiliano Pagani, Sergio Abrignani, Stefano Biffo
RESUMEN

Naive T cells respond to T cell receptor (TCR) activation by leaving quiescence, remodeling metabolism, initiating expansion, and differentiating toward effector T cells. The molecular mechanisms coordinating the naive to effector transition are central to the functioning of the immune system, but remain elusive. Here, we discover that T cells fulfill this transitional process through translational control. Naive cells accumulate untranslated mRNAs encoding for glycolysis and fatty acid synthesis factors and possess a translational machinery poised for immediate protein synthesis. Upon TCR engagement, activation of the translational machinery leads to synthesis of GLUT1 protein to drive glucose entry. Subsequently, translation of ACC1 mRNA completes metabolic reprogramming toward an effector phenotype. Notably, inhibition of the eIF4F complex abrogates lymphocyte metabolic activation and differentiation, suggesting ACC1 to be a key regulatory node. Thus, our results demonstrate that translation is a direct mediator of T cell metabolism and indicate translation factors as targets for novel immunotherapeutic approaches.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Anti-β-actina monoclonal antibody produced in mouse, clone AC-74, purified immunoglobulin, buffered aqueous solution
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Número de referencia del producto (SKU)Tamaño de envaseDisponibilidadPrecio
100 mL
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
HUF 13,500.00

To order products, please Contacto your local dealer.

Sigma-Aldrich
Anti-puromicina, clon 12D10, anticuerpo conjugado Alexa Fluor 488, clone 12D10, 0.5 mg/mL, from mouse
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Número de referencia del producto (SKU)Tamaño de envaseDisponibilidadPrecio
100 mL
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
HUF 13,500.00

To order products, please Contacto your local dealer.

Sigma-Aldrich
Anti-Vinculin Antibody, clone V284, clone V284, Upstate®, from mouse
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Número de referencia del producto (SKU)Tamaño de envaseDisponibilidadPrecio
100 mL
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
HUF 13,500.00

To order products, please Contacto your local dealer.