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Merck

Affinity adsorbent based on combinatorial phage display peptides that bind alpha-cobratoxin.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2004-05-12)
Woo-Hyeon Byeon, Bernard Weisblum
RESUMEN

Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to agarose through the alpha-amino terminus. Such affinity chromatography supports selectively bound the alpha-cobratoxin component from crude venom, while passage of the crude venom over the support selectively depleted the venom of this component. The selective binding of alpha-cobratoxin to peptide-based solid-phase supports suggests that a limitless variety of peptides similarly obtained by combinatorial phage display can be used to craft specific analytical and preparative tools.

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Sigma-Aldrich
N-Hydroxysuccinimidyl-Sepharose 4 Fast Flow, matrix fast-flow highly cross-linked 4% beaded agarose