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T2391

Sigma-Aldrich

Triosephosphate Isomerase from rabbit muscle

Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

Sinónimos:

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

Número de CAS:
9023-78-3
Comisión internacional de enzimas:
5.3.1.1 (BRENDA, IUBMB)
MDL number:
MFCD00082080
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

rabbit muscle

Quality Level

200

type

Type III-S

form

ammonium sulfate suspension

specific activity

≥4,000 units/mg protein

mol wt

calculated mol wt 53.2 kDa

foreign activity

3-phosphoglyceric phosphokinase ≤0.01%
glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001%

storage temp.

2-8°C

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Application

Triosephosphate isomerase has been used in a study to assess molecular characterizations of Cryptosporidium, Giardia, and Enterocytozoon. Triosephosphate isomerase has also been used in a study to investigate apoptosis of hepatocellular carcinoma cell lines.

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Yi-Lin Sophia Chen et al.
Peptides, 36(2), 257-265 (2012-06-05)
Due to its malignancy, the development of effective therapeutic strategies for hepatocellular carcinoma (HCC) is of urgent needs. Natural antimicrobial peptides (AMPs), also known as host defense peptides (HDPs), not only act as direct antimicrobial agents, but also represent important
Beatty V Maikai et al.
Experimental parasitology, 131(4), 452-456 (2012-06-06)
The use of molecular diagnostic tools in epidemiological investigations of Cryptosporidium, Giardia, and Enterocytozoon has provided new insights into their diversity and transmission pathways. In this study, 157 stool specimens from 2-month to 70-year-old patients were collected, a polymerase chain
Susan K Boehlein et al.
The Plant journal : for cell and molecular biology, 99(1), 23-40 (2019-02-13)
Cereal yields decrease when grain fill proceeds under conditions of prolonged, moderately elevated temperatures. Endosperm-endogenous processes alter both rate and duration of dry weight gain, but underlying mechanisms remain unclear. Heat effects could be mediated by either abnormal, premature cessation
Tiia Kittilä et al.
Chembiochem : a European journal of chemical biology, 17(7), 576-584 (2016-01-12)
Nonribosomal peptide synthetases (NRPSs) produce many important and structurally complex natural products. Because of their architectures, reprogramming NRPSs has long been attempted to access new bioactive compounds. However, detailed characterization of NRPS catalysis and substrate selectivity by adenylation (A) domains
Rebecca Blomberg et al.
Nature, 503(7476), 418-421 (2013-10-18)
Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of
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Artículos

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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