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Merck

SCJ4600022

Sigma-Aldrich

CF 555 succinimidyl ester

suitable for fluorescence, ≥90% (TLC)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

assay

≥90% (TLC)

form

solid

mol wt

~959 g/mol (free acid)

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

ε (extinction coefficient)

150000 at 555 nm

absorbance ratio

A280/555 nm 0.08

fluorescence

λex 488 or 532 nm (Flow cytometry laser line)
λex 488 or 532 nm (Microscopy laser line)
λex 555 nm; λem 565 nm

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

General description

CF 555 succinimidyl ester (CF555 SE) is used for labeling proteins or other biomolecules having an amine group. The succinimidyl ester group of the dye reacts with an amine group to form a stable amide linkage.

Application

CF 555 succinimidyl ester has been used to label concanavalin A for fluorescence resonance energy transfer (FRET) experiments.

Features and Benefits

1. Has comparable brightness and photostability as Cy®3 and Alexa Fluor555. 2.Highly water-soluble. 3. pH-insensitive.

Preparation Note

Protect from light and moisture

Reconstitution

Dissolve in DMSO

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.
Cy is a registered trademark of Cytiva
Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Yongju Kim et al.
Chemistry, an Asian journal, 14(7), 952-957 (2019-02-20)
Here we report fluorescence turn-on synthetic lipid rafts by self-assembly of a cationic distyrylanthracene derivative on a negatively-charged sheet in an aqueous solution. First, the negatively-charged 2D membrane structure is formed by lateral associations of aromatic rods with carboxylate groups.

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