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Key Documents

S6782

Sigma-Aldrich

Superdex®

Superdex®200 Prep Grade

Sinónimos:

Size exclusion media, Superdex column

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About This Item

MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

form

suspension (20% aqueous ethanol)

technique(s)

protein array: suitable

matrix

cross-linked agarose

particle size

24-44 μm

pore size

1-100 kDa MW range (dextrans)
10-600 kDa MW range (proteins)

operating pH range

1-14(short term)
3-12(long term)

storage temp.

2-8°C

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General description

Combina la elevada selectividad de una matriz Sefadex con la estabilidad física y química de la agarosa reticulada.

Application

Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.
Optimizada para separaciones preparativas para alta resolución.

Legal Information

Superdex is a registered trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

104.0 °F

flash_point_c

40 °C


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J Oh et al.
Human molecular genetics, 9(3), 375-385 (2000-02-03)
Hermansky-Pudlak syndrome (HPS) is a rare autosomal recessive disorder in which oculocutaneous albinism, bleeding tendency and a ceroid-lipofuscin lysosomal storage disease result from defects of multiple cytoplasmic organelles: melanosomes, platelet dense granules and lysosomes. The HPS polypeptide, a 700 amino
J L Van Hove et al.
Biochemistry and molecular biology international, 43(3), 613-623 (1997-11-14)
Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A
Mario Jiz et al.
Infection and immunity, 76(7), 3164-3169 (2008-04-23)
Despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. Rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine
Thomas Ouellette et al.
Protein expression and purification, 30(2), 156-166 (2003-07-26)
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by homogenization with 6
Purification of sucrose synthase from rice and its protein-chemical characterization.
Elling, L., and Kula, M.-R.
Journal of Biotechnology, 29(3), 277-286 (1993)

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