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Key Documents

GE28-9889-53

Sigma-Aldrich

XK 50/30 Column

Sinónimos:

XK 50/30 Column

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About This Item

UNSPSC Code:
41115711
NACRES:
SB.52

technique(s)

LPLC: suitable

General description

XK empty columns are designed for liquid chromatography at low to medium pressure. The user-friendly design ensures trouble-free operation with fittings for direct connection to ÄKTA design instruments or other high-performance liquid chromatography systems. The product family is based on a robust design for high reproducibility and accurate results. XK columns are compatible with aqueous solutions and most organic solvents used in liquid chromatography of macromolecules.
XK empty columns provide reliable and reproducible separations. The tube material is of borosilicate glass, which allows for visual inspection of the media bed. The columns are made of biocompatible materials that exhibit excellent chemical resistance. A broad range of column dimensions makes XK columns suitable for many different purification needs. Column packing can be performed using either a packing reservoir or an extra column tube attached with a packing connector. The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments of the bed height, disassembly, and cleaning. The design of the adapter plunger gives a uniform flow, which maintains the integrity of the packed bed during operations. An extensive range of spare parts is available, facilitating maintenance.

Features and Benefits

Adapter QuickLock mechanism that facilitates column handling and cleaning
Cooling jacket for sensitive purifications
Fittings for direct connection to chromatographiy systems
Plunger design to ensure a homogeneous buffer distribution and reproducible packing
User friendly and robust empty columns, designed for liquid chromatography at low to medium pressure.

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocolos

This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

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