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D4668

Sigma-Aldrich

Dextranase from Penicillium sp.

lyophilized powder, 100-250 units/mg protein

Sinónimos:

1,6-α-D-Glucan 6-glucanohydrolase

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About This Item

Número de CAS:
9025-70-1
Comisión internacional de enzimas:
3.2.1.11 (BRENDA, IUBMB)
EC Number:
232-803-9
MDL number:
MFCD00130940
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

100-250 units/mg protein

composition

Protein, ~25% Lowry

storage temp.

2-8°C

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Application

Dextranase from Penicillium sp. can be used to modify alternan. Dextranase from Penicillium sp. has also been used in a study that explored the possibility that the Dex protein might adopt a fold unfamiliar to any known structure.

Biochem/physiol Actions

The product is an endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

Unit Definition

One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.

Physical form

Partially purified, lyophilized powder

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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T Pons et al.
Proteins, 31(4), 345-354 (1998-06-17)
The DEX gene encodes an extracellular dextranase (EC 3.2.1.11); this enzyme hydrolyzes the alpha(1,6) glucosidic bond contained in dextran to release small isomaltosaccharides. Sequence analysis has revealed only one homologous sequence, CB-8 protein, from Arthrobacter sp., with 30% sequence identity.
Timothy D Leathers et al.
Biotechnology letters, 31(2), 289-293 (2008-10-22)
Alternan is a unique glucan with a backbone structure of alternating alpha-(1 --> 6) and alpha-(1 --> 3) linkages. Previously, we isolated strains of Penicillium sp. that modify native, high molecular weight alternan in a novel bioconversion process to a
Dextranase in sugar industry: A review
Efrain Rodriguez Jimenez
Sugar Tech, 11(2), 124-134 (2009)
Controlled nanoparticle formation by enzymatic deshelling of biopolymer-stabilized nanosuspensions.
Yi-Yeoun Kim et al.
Small (Weinheim an der Bergstrasse, Germany), 5(8), 913-918 (2009-02-11)
Akira Yano et al.
Applied microbiology and biotechnology, 86(2), 615-623 (2009-11-11)
Mushrooms contain large quantities of alpha-glucans. Shiitake (Lentinula edodes), Japan's most popular edible mushroom, has been reported to contain about 6% (weight/dried weight) of alpha-(1,3)-glucan. This glucan is one of the major components of oral biofilm formed by the cariogenic
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