C1224
Caspase 3 human
≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein
Sinónimos:
Apopain, CPP32, Yama
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32
Productos recomendados
recombinant
expressed in E. coli (C-terminal histidine-tagged)
Quality Level
assay
≥90% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
≥1.0 units/mg protein
mol wt
~30 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CASP3(836)
¿Está buscando productos similares? Visita Guía de comparación de productos
General description
Research area: Apoptosis
Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.
Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.
Application
Caspase 3 human has been used to digest human r-vimentin. It has also been used in caspase-3 inhibition assay and protease inhibition assay.
This product may also be used in cell-based apoptosis assay.
This product may also be used in cell-based apoptosis assay.
Biochem/physiol Actions
Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.
Unit Definition
One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp-pNA per min at pH 7.4 at 25 °C.
Physical form
Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS
Preparation Note
Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits.
Related product
Referencia del producto
Descripción
Precios
Substrate
Referencia del producto
Descripción
Precios
antibody
Referencia del producto
Descripción
Precios
inhibitor
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Cross-presentation of caspase-cleaved apoptotic self antigens in HIV infection
Rawson PM, et al.
Nature Medicine, 13(12), 1431-1431 (2007)
A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells
Hight MR, et al.
Clinical Cancer Research, 20(8), 2126-2135 (2014)
Fernando Garces et al.
Cell reports, 30(6), 1714-1723 (2020-02-13)
Calcitonin-gene-related peptide (CGRP) plays a key role in migraine pathophysiology. Aimovig (erenumab; erenumab-aooe in the United States) is the only US Food and Drug Administration (FDA)-approved monoclonal antibody (mAb) therapy against the CGRP receptor (CGRPR) for the prevention of migraine.
J Fuller et al.
The Journal of general virology, 100(3), 392-402 (2019-02-06)
The Nairoviridae family within the Bunyavirales order comprise tick-borne segmented negative-sense RNA viruses that cause serious disease in a broad range of mammals, yet cause a latent and lifelong infection in tick hosts. An important member of this family is
Deju Ye et al.
Nature chemistry, 6(6), 519-526 (2014-05-23)
Directed self-assembly of small molecules in living systems could enable a myriad of applications in biology and medicine, and already this has been used widely to synthesize supramolecules and nano/microstructures in solution and in living cells. However, controlling the self-assembly
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico