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Merck

B5525

Sigma-Aldrich

Bromophenol Blue sodium salt

Sinónimos:

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

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About This Item

Fórmula empírica (notación de Hill):
C19H9Br4NaO5S
Número de CAS:
Peso molecular:
691.94
Beilstein/REAXYS Number:
6995701
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.52

form

powder

technique(s)

electrophoresis: suitable

solubility

water: soluble

suitability

suitable for nucleic acid electrophoresis

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Related product

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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