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A7310

Sigma-Aldrich

Anti-Water Channel Aquaporin 2 antibody produced in rabbit

IgG fraction of antiserum, lyophilized powder

Sinónimos:

Anti-Aquaporin 2, Aquaporin 2 Detection, Rabbit Anti-Aquaporin 2

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About This Item

MDL number:
MFCD02262543
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable
western blot (chemiluminescent): 1:200-1:1000

UniProt accession no.

P41181

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AQP2(359)
mouse ... Aqp2(11827)
rat ... Aqp2(25386)

General description

Aquaporin 2 (AQP2) exists as a tetramer with C terminus showing multiple conformations and binds two cadmium ions. AQP2 gene is mapped to human chromosome 12q13.12. It is associated with intracellular vesicles.
Aquaporin 2 (AQP2) is a water channel protein that regulates water balance in renal connecting tubules and collecting ducts. AQP2 is activated by anti-diuretic hormone and subsequently facilitates water inflow in renal cells. This protein also interacts with integrin to modulate epithelial cell morphogenesis and cell movement . Anti-Water Channel Aquaporin 2 antibody recognizes aquaporin 2 from rat by immunoblotting. The antibody also reacts with mouse and human AQP2.

Immunogen

synthetic peptide corresponding to amino acids 254-271 of rat or mouse aquaporin 2 (with additional N-terminal lysine and tyrosine) conjugated to KLH with glutaraldehyde. The epitope is identical in rat and mouse and highly conserved in human.

Application

Anti-Water Channel Aquaporin 2 antibody is also suitable for use in chemiluminescent western blot (1:200-1:1000), and immunoblot.
Anti-Water Channel Aquaporin 2 antibody produced in rabbit has been used in:
  • immunostaining
  • immunohistochemistry
  • immunofluorescence
  • immunoblotting

Immunohistochemistry was performed on 10% formalin-fixed parraffin embedded rat kidney sections using rabbit anti-AQP2 at a concentration of 5μg/ml. Use of anti-AQP2 allowed visualization of the medullary thick ascending limb and collecting ducts.

Biochem/physiol Actions

Aquaporin (AQP2) N-terminal region plays a key role in trafficking. The C-terminal phosphorylation is crucial for the water permeability function of the apical membrane. Also, ubiquitination at lysine 270 is needed for AQP2 sorting and degradation in lysosomes or elimination through exosomes. Mutation in AQP2 gene is implicated in congenital nephrogenic diabetes insipidus (NDI), a water balance disorder. The functional dysregulation of AQP2 is also correlated to preeclampsia, congestive heart failure and liver cirrhosis.

Physical form

Lyophilized from a solution containing phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide.

Preparation Note

Isolated on immobilized Protein A

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Alexandra Rieger et al.
PloS one, 11(7), e0158977-e0158977 (2016-07-16)
During nephrogenesis, POU domain class 3 transcription factor 3 (POU3F3 aka BRN1) is critically involved in development of distinct nephron segments, including the thick ascending limb of the loop of Henle (TAL). Deficiency of POU3F3 in knock-out mice leads to
Kuniaki Takata et al.
Histochemistry and cell biology, 130(2), 197-209 (2008-06-21)
Aquaporins (AQPs) are membrane proteins serving in the transfer of water and small solutes across cellular membranes. AQPs play a variety of roles in the body such as urine formation, prevention from dehydration in covering epithelia, water handling in the
Khalil El Karoui et al.
Nature communications, 7, 10330-10330 (2016-01-21)
In chronic kidney disease (CKD), proteinuria results in severe tubulointerstitial lesions, which ultimately lead to end-stage renal disease. Here we identify 4-phenylbutyric acid (PBA), a chemical chaperone already used in humans, as a novel therapeutic strategy capable to counteract the
Novel AVPR2 variant in a male infant with nephrogenic diabetes insipidus who showed delayed head control
Hasegawa K, et al.
Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology, 28(4), 155-158 (2019)
Hydrochlorothiazide ameliorates polyuria caused by tolvaptan treatment of polycystic kidney disease in PCK rats
Wang A, et al.
Clinical and Experimental Nephrology, 23(4), 455-464 (2019)
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