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Merck

83400

Millipore

D-(+)-Rafinosa pentahydrate

suitable for microbiology, ≥99.0%, Microbiological media component

Sinónimos:

O-α-D-Galactopiranosil-(1→6)-α-D-glucopiranosil β-D-fructofuranósido, Melitosa, Melitriosa

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About This Item

Fórmula empírica (notación de Hill):
C18H32O16 · 5H2O
Número de CAS:
Peso molecular:
594.51
Beilstein/REAXYS Number:
99541
EC Number:
MDL number:
UNSPSC Code:
41106212
PubChem Substance ID:
NACRES:
NA.85

Quality Level

assay

≥99.0% (HPLC)
≥99.0%

form

powder

optical activity

[α]20/D +105±2°, c = 10% in H2O

ign. residue

≤0.1% (as SO4)

mp

78-80 °C

solubility

H2O: 0.1 g/mL, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

As: ≤0.1 mg/kg
Ca: ≤20 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

application(s)

microbiology

storage temp.

10-30°C

SMILES string

O.O.O.O.O.OC[C@H]1O[C@H](OC[C@H]2O[C@H](O[C@]3(CO)O[C@H](CO)[C@@H](O)[C@@H]3O)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C18H32O16.5H2O/c19-1-5-8(22)11(25)13(27)16(31-5)30-3-7-9(23)12(26)14(28)17(32-7)34-18(4-21)15(29)10(24)6(2-20)33-18;;;;;/h5-17,19-29H,1-4H2;5*1H2/t5-,6-,7-,8+,9-,10-,11+,12+,13-,14-,15+,16+,17-,18+;;;;;/m1...../s1

InChI key

BITMAWRCWSHCRW-PFQJHCPISA-N

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General description

Raffinose is a natural trisaccharide composed of glucose, galactose, and fructose. It is a water-soluble oligosaccharide, most commonly present in legumes but also in sugar beets and remains during the process in the molasse. It is primarily used as a carbon source in the cultivation of yeast but also has certain cell proliferative and anti-oxidative properties that can prove useful in culture media. Raffinose is used by beneficial probiotic bacteria and an essential component of certain cryoprotectant solutions.

Application

D-(+)-Raffinose pentahydrate can be used as a carbon source for the cultivation of yeast
It is used by beneficial probiotic bacteria like Bifidobacteria and Lactobacilli but could be used as well for differentiation and identification of Escherichia coli, Enterococcus faecium and Streptococcus pneumoniae.
It can also be used in certain cryoprotectant solutions.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Poonam Bheda et al.
STAR protocols, 1(3), 100228-100228 (2020-12-31)
The budding yeast Saccharomyces cerevisiae is an excellent model organism to dissect the maintenance and inheritance of phenotypes due to its asymmetric division. This requires following individual cells over time as they go through divisions to define pedigrees. Here, we
Vidhya Bharathi et al.
Bio-protocol, 7(15), e2440-e2440 (2017-08-05)
The yeast Saccharomyces cerevisiae (S. cerevisiae) harboring ade1 or ade2 mutations manifest red colony color phenotype on rich yeast medium YPD. In these mutants, intermediate metabolites of adenine biosynthesis pathway are accumulated. Accumulated intermediates, in the presence of reduced glutathione
Poonam Bheda et al.
Molecular cell, 78(5), 915-925 (2020-05-12)
Transcriptional memory of gene expression enables adaptation to repeated stimuli across many organisms. However, the regulation and heritability of transcriptional memory in single cells and through divisions remains poorly understood. Here, we combined microfluidics with single-cell live imaging to monitor
Antonio Marsella et al.
Cell reports, 34(13), 108906-108906 (2021-04-01)
The Mre11-Rad50-Xrs2 (MRX) complex detects and processes DNA double-strand breaks (DSBs). Its DNA binding and processing activities are regulated by transitions between an ATP-bound state and a post-hydrolysis cutting state that is nucleolytically active. Mre11 endonuclease activity is stimulated by

Artículos

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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