Saltar al contenido
Merck

11442074001

Roche

BM-Purple

Roche, pkg of 100 mL, solution

Sinónimos:

chromogenic substrate

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352204

form

solution

Quality Level

packaging

pkg of 100 mL

manufacturer/tradename

Roche

color

dark purple

solubility

H2O: insoluble

shipped in

wet ice

storage temp.

2-8°C

General description

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

Application

  • Colorimetric AP substrate for: Immunohistocytochemistry
  • Western blot
  • Southern blot
  • Northern blot
  • Colony and plaque hybridization
  • In situ hybridization

Components

Ready-to-use solution.

Preparation Note

Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.
Working solution: Solubility in water: insoluble.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Valentina Garcia-Lee et al.
The International journal of developmental biology, 65(4-5-6), 395-401 (2020-09-16)
The cell differentiation of the musculoskeletal system is highly coordinated during limb development. In the distal-most region of the limb, WNT and FGF released from the apical ectodermal ridge maintain mesenchymal cells in the undifferentiated stage. Once the cells stop
Christoph Kessle
Nonradioactive Analysis of Biomolecules null
Jonas Malkmus et al.
Nature communications, 12(1), 5557-5557 (2021-09-23)
Precise cis-regulatory control of gene expression is essential for normal embryogenesis and tissue development. The BMP antagonist Gremlin1 (Grem1) is a key node in the signalling system that coordinately controls limb bud development. Here, we use mouse reverse genetics to
Ho Chi Leung et al.
Zygote (Cambridge, England), 1-12 (2021-09-18)
It has previously been reported that in ex vivo planar explants prepared from Xenopus laevis embryos, the intracellular pH (pHi) increases in cells of the dorsal ectoderm from stage 10.5 to 11.5 (i.e. 11-12.5 hpf). It was proposed that such
Deepika Sharma et al.
eLife, 10 (2021-10-15)
Osteoblast differentiation is sequentially characterized by high rates of proliferation followed by increased protein and matrix synthesis, processes that require substantial amino acid acquisition and production. How osteoblasts obtain or maintain intracellular amino acid production is poorly understood. Here, we

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico