CBL171-I
Anti-α-Smooth Muscle Actin (ACTA2) Antibody,
mouse monoclonal, ASM-1 (1A4)
Sinónimos:
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
About This Item
Productos recomendados
Nombre del producto
Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4, clone ASM-1 (1A4), from mouse
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
ASM-1 (1A4), monoclonal
reactividad de especies
mouse, chicken, rat, human, bovine
reactividad de especies (predicha por homología)
equine (based on 100% sequence homology)
técnicas
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotipo
IgG2aκ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ACTA2(59)
Descripción general
Especificidad
Inmunógeno
Aplicación
Cell Structure
Cytoskeletal Signaling
Western Blotting Analysis: A representative lot detected smooth muscle actin in PC-3M-LN4 and PC-3M-LN4-derived human prostate cancer cells (Zvieriev, V., et al. (2005). Biochem. Biophys. Commun. 337(2):498-504).
Western Blotting Analysis: A representative lot detected developing stage-dependent vascular alpha-actin level in chicken heart of various somite stages (Ehler, E., et al. (2004). Dev. Dyn. 229(4):745-755).
Western Blotting Analysis: A representative lot detected aortic actin, but not actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), or myocardium (alpha-myocardial) in various human, rat, bovine and rabbit tissue and cell lysates (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
Immunohistochemistry Analysis: A representative lot immunostained smooth muscle actin-positive myofibroblasts in paraformaldehyde-fix rat skin wounds sections (Mirastschijski, U., et al. (2010). Wound Repair Regen. 18(2):223-234).
Immunohistochemistry Analysis: A representative lot immunostained activated myofibroblasts in formalin-fixed, paraffin-embedded mouse submandibular salivary gland (Hall, B.E., et al. (2010). Lab. Invest. 90(4):543-555).
Immunohistochemistry Analysis: A representative lot immunostained α-SMA-positive cells in paraffin-embedded rat healing patellar tendon sections (Fu, S.C., et al. (2008). J. Orthop. Res. 26(3):374-383).
Immunocytochemistry Analysis: Representative lots immunostained the mesoderm layer of embryoid bodies formed in vitro from northern white rhinoceros iPSCs, as well as murine iPSCs and mESCs (Ben-Nun, I.F., et al. (2011). Nat. Methods. 8(10):829-831; Gerlach, J.C., et al. (2010). Cells Tissues Organs. 192(1):39-49; Shao, L., et al. (2009). Cell Res. 19(3):296-306).
Immunohistochemistry Analysis: A representative lot immunostained a subsets of cultured rat aortic medial smooth muscle cells (SMCs) and dermal fibroblasts (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
Immunohistochemistry Analysis: A representative lot immunostained cultured rat tendon cells (Fu, S.C., et al. (2008). J. Orthop. Res. 26(3):374-383).
Immunofluorescence Analysis: A representative lot immunostained myofibrils using methanol-fixed, 9-somite stage chicken heart whole-mount preparations. The vascular alpha-actin staining started to disappear from a subset of the myofibrils when using 10-somite stage chicken heart preparations (Ehler, E., et al. (2004). Dev. Dyn. 229(4):745-755).
Immunofluorescence Analysis: A representative lot immunostained stromal vessel SM cells in acetone-fixed benign (leiomyomas) and malignant (leiomyosarcomas & intravascular leiomyomatosis) smooth muscle (SM) neoplasms cryosections (Schürch, W., et al. (1987). Am. J. Pathol. 128(1):91-103).
Immunofluorescence Analysis: A representative lot immunostained chicken gizzard and rat myocardium blood vessels, as well as smooth muscle cells (SMCs) in various acetone-fixed human and rat cryosections, whereas chicken gizzard parenchyrnal cells, rat cardiocytes, aorta endothelial cells and adventitial fibroblasts were negative (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
ELISA Analysis: A representative lot detected aortic actin, but not actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), or myocardium (alpha-myocardial) by ELISA (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
Calidad
Western Blotting Analysis: 0.5 µg/mL of this antibody detected smooth muscle actin in 10 µg of HUVEC lysate.
Descripción de destino
Forma física
Almacenamiento y estabilidad
Otras notas
Cláusula de descargo de responsabilidad
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico