GC Analysis of Diphenylamine and Methoxychlor in Apples on a 14% Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

homogenize 50 g sample in 100 mL acetonitrile then add 10 g NaCl and homogenize 5 min

SPE tube/cartridge

ENVI-Carb, 500 mg/6 mL

column

14% cyanopropylphenyl polysiloxane, 30 m × 0.25 mm I.D., 0.15 μm (Supelco equivalent, Equity-1701, 28372-U)

oven

70 °C (2 min), 25 °C/min to 130 °C, 2 °C/min to 220 °C, 10 °C/min to 280 °C (4.6 min)

detector

MSD, 285 °C

carrier gas

helium

injection

2 μL splitless