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  • IL-10-producing CD4(+) T cells negatively regulate fucosylation of epithelial cells in the gut.

IL-10-producing CD4(+) T cells negatively regulate fucosylation of epithelial cells in the gut.

Scientific reports (2015-11-03)
Yoshiyuki Goto, Aayam Lamichhane, Mariko Kamioka, Shintaro Sato, Kenya Honda, Jun Kunisawa, Hiroshi Kiyono
ABSTRACT

Fucosylated glycans on the surface of epithelial cells (ECs) regulate intestinal homeostasis by serving as attachment receptors and a nutrient source for some species of bacteria. We show here that epithelial fucosylation in the ileum is negatively regulated by IL-10-producing CD4(+) T cells. The number of fucosylated ECs was increased in the ileum of mice lacking T cells, especially those expressing αβ T cell receptor (TCR), CD4, and IL-10. No such effect was observed in mice lacking B cells. Adoptive transfer of αβTCR(+) CD4(+) T cells from normal mice, but not IL-10-deficient mice, normalized fucosylation of ECs. These findings suggest that IL-10-producing CD4(+) T cells contribute to the maintenance of the function of ECs by regulating their fucosylation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Interleukin-10 from rat, >95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
Sigma-Aldrich
Interleukin-10 human, ≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
Sigma-Aldrich
Interleukin-10 from mouse, >97% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture
Sigma-Aldrich
IL-10 human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sigma-Aldrich
IL-10 human, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)