Skip to Content

End of year dates: Orders must be billed by 13/12. Merck Argentina will be closed from 23/12 to 02/01.

Merck

Assembly of phagocyte NADPH oxidase: A concerted binding process?

Biochimica et biophysica acta (2014-08-12)
Gilda Karimi, Chantal Houée Levin, Marie Claire Dagher, Laura Baciou, Tania Bizouarn
ABSTRACT

The phagocyte NADPH-oxidase is a multicomponent enzyme that generates superoxide anions. It comprises a membrane redox component flavocytochrome b558 and four cytosolic proteins (p67(phox), p47(phox), p40(phox) and Rac) that must assemble to produce an active system. In this work we focused on the spatio-temporal control of the activation process of phagocyte NADPH oxidase. A wide range of techniques including fast kinetics with a stopped-flow apparatus and various combinations of the activating factors was used to test the order of assembly and the role of the p47(phox)-p67(phox) complex. The data presented here are consistent with the absence of a catalytic role of the p47(phox)-p67(phox) interacting state and support the idea of independent binding sites for the cytosolic proteins on the flavocytochrome b558 allowing random binding order. However, the formation of the active complex appears to involve a synergistic process of binding of the activated cytosolic subunits to cytochrome b558. All partners should be in the vicinity for optimal assembly, a delay or the absence of one of the partners in this process seems to lead to a decrease in the efficiency of the catalytic core. The activation and assembly of the NADPH oxidase components have to be achieved simultaneously for the formation of an efficient and optimal enzyme complex. This mechanism appears to be incompatible with continuous fast exchanges of the cytosolic proteins during the production of superoxide ion in the phagosome.

MATERIALS
Product Number
Brand
Product Description

SAFC
BIS-TRIS
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
BIS-TRIS, BioUltra, ≥99.0% (NT)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
BIS-TRIS, BioXtra, ≥98.0% (titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
BIS-TRIS, ≥98.0% (titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
BIS-TRIS, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Supelco
Glutathione, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

SAFC
BIS-TRIS
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Glutathione, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
Arachidonic acid, from non-animal source, ≥98.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
Arachidonic acid, >95.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
L-Glutathione reduced, BioXtra, ≥98.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
L-Glutathione reduced, suitable for cell culture, BioReagent, ≥98.0%, powder
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
L-Glutathione reduced, ≥98.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.

Sigma-Aldrich
Kit for Molecular Weights 14,000-500,000 Non-denaturing
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPrice
50 mL
1 Estimated to ship on February 22, 2025
$421.00

To order products, please contact your local dealer.