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  • Growth and hematology of juvenile pacu Piaractus mesopotamicus (Holmberg 1887) fed with increasing levels of vitamin E (DL-α-tocopheryl acetate).

Growth and hematology of juvenile pacu Piaractus mesopotamicus (Holmberg 1887) fed with increasing levels of vitamin E (DL-α-tocopheryl acetate).

Anais da Academia Brasileira de Ciencias (2013-03-30)
Ricardo Y Sado, Alvaro J A Bicudo, José E P Cyrino
ABSTRACT

Intensive fish production systems are characterized by 100% artificial feeding, so any dietary imbalances or deficiencies may lead to diseases outbreaks and economic losses. This study was set out to determine the effects of increasing levels of dietary vitamin E on growth and hematology of juvenile pacu. Fishes were fed for 90 days, twice a day until apparent satiation with semi-purified diets containing 0.0; 25; 50; 150; 300 or 600 mg.kg-1 diet DL-α-tocopheryl acetate in a completely randomized design trial (n=4); biometrical and hematological data were collected and analyzed. Fishes fed with vit E diet (150 mg.kg-1) showed higher (p<0.05) weight gain and specific growth. Hematocrit, erythroblast number and total plasma protein were increased (p<0.05) in fishes fed diet with no vit E diet. Vitamin E supplementation in artificial diets for pacu is essential for growth and maintenance of normal erythropoiesis.

MATERIALS
Product Number
Brand
Product Description

Supelco
DL-alpha-Tocopherol acetate, analytical standard
Sigma-Aldrich
(+)-α-Tocopherol, from vegetable oil, Type V, ~1000 IU/g
Sigma-Aldrich
(+)-α-Tocopherol, Type VI, from vegetable oil, liquid (≥0.88M based on potency, density and molecular wt.), BioReagent, suitable for insect cell culture, ≥1000 IU/g
Sigma-Aldrich
DL-α-Tocopherol acetate, ≥96% (HPLC)
α-Tocopherol acetate, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
DL-α-Tocopherol acetate, tested according to Ph. Eur.
Supelco
Tocopheryl Acetate, a, Pharmaceutical Secondary Standard; Certified Reference Material
All-rac-alpha-tocopheryl acetate for peak identification, European Pharmacopoeia (EP) Reference Standard