Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Journal of agricultural and food chemistry (2004-08-19)

Dawn M Chapman, John H Thorngate, Mark A Matthews, Jean-Xavier Guinard, Susan E Ebeler

ABSTRACT

A rapid and automated solid phase microextraction (SPME) stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for 2-methoxy-3-isobutylpyrazine (MIBP) quantification in red wine was developed. Wines with 30% (w/v) NaCl and 2-methoxy-(2)H(3)-3-isobutylpyrazine internal standard were sampled with a 2 cm divinylbenzene/carboxen/poly(dimethylsiloxane) SPME fiber for 30 min at 40 degrees C and analyzed by GC-MS. The method was used to measure MIBP concentrations in Cabernet Sauvignon wines that were produced from six winter pruning treatments over two vintages. MIBP concentrations were significantly negatively correlated with buds per vine. In addition, the MIBP concentration was directly related to sensory vegetal intensity ratings obtained by descriptive analysis.

MATERIALS

Product Number

Brand

Product Description

57912-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 1 cm

11072-U

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

57906-U

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, for use with autosampler, needle size 23 ga, metal alloy fiber

57914-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm

54041-U

Supelco

Carboxen^® 1000 Reversible SPE Tube, 200 mg/mL, pk 30

11048-U

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 569, 20-45 mesh, bottle of 500 g

57299-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm

57298-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler

57295-U

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler

57343-U

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler

10477-U

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 1000, 40-60 mesh, bottle of 50 g

57335-U

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler

57328-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder

10478-U

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

25467

Supelco

Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

10269

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 569, 20-45 mesh, bottle of 10 g

57348-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, StableFlex

24246

Supelco

Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

25461

Supelco

Carboxen^®-1006 PLOT Capillary GC Column, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

10263

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 563, 20-45 mesh, bottle of 10 g

11324-U

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 564, 20-45 mesh, pack of 144 × 290 mg

10264

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 564, 20-45 mesh, bottle of 10 g

10471

Supelco

Carboxen^® Adsorbent, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

57318

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

57334-U

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber

57319

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler

57329-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler

57344-U

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder

24241-U

Supelco

Carboxen^®-1006 PLOT Capillary GC Column, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

23446-U

Supelco

ORBO^™-93 Sorbent Tube, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea