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  • Titanium coating with mussel inspired polymer and bio-orthogonal chemistry enhances antimicrobial activity against Staphylococcus aureus.

Titanium coating with mussel inspired polymer and bio-orthogonal chemistry enhances antimicrobial activity against Staphylococcus aureus.

Materials science & engineering. C, Materials for biological applications (2020-08-19)
Magdalena Czuban, Michaël W Kulka, Lei Wang, Anna Koliszak, Katharina Achazi, Christoph Schlaich, Ievgen S Donskyi, Mariagrazia Di Luca, Jose M Mejia Oneto, Maksim Royzen, Rainer Haag, Andrej Trampuz
ABSTRACT

Implant-associated infections present severe and difficult-to-treat complications after surgery, related to implant biofilm colonization. Systemic administration of antibiotics cannot reach sufficient concentrations at the infected site and may be toxic. Here we describe how mussel-inspired dendritic material coated on a titanium surface can locally activate a prodrug of daptomycin (pro-dapto) to treat methicillin-resistant Staphylococcus aureus. The mechanism of the prodrug activation is based on bio-orthogonal click chemistry between a tetrazine (Tz) and trans-cyclooctene (TCO). The former is attached to the dendritic polymer, while the later converts daptomycin into a prodrug. Characterization of the material's properties revealed that it is hydrophobic, non-toxic, and stable for a prolonged period of time. We envision that the titanium coated dendritic material will be able to improve the treatment of implant-associated infections by concentrating systemically administered antibiotic prodrugs, thus converting them into active localized medicines.

MATERIALS
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Daptomycin, cyclic lipopeptide antibiotic
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MRSA Selective Supplement, suitable for microbiology
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{2-[2-(Fmoc-amino)ethoxy]ethoxy}acetic acid, ≥95.0% (HPLC)
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