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  • Comparative Evaluation of Broth Microdilution with Polystyrene and Glass-Coated Plates, Agar Dilution, E-Test, Vitek, and Disk Diffusion for Susceptibility Testing of Colistin and Polymyxin B on Carbapenem-Resistant Clinical Isolates of Acinetobacter baumannii.

Comparative Evaluation of Broth Microdilution with Polystyrene and Glass-Coated Plates, Agar Dilution, E-Test, Vitek, and Disk Diffusion for Susceptibility Testing of Colistin and Polymyxin B on Carbapenem-Resistant Clinical Isolates of Acinetobacter baumannii.

Microbial drug resistance (Larchmont, N.Y.) (2018-02-07)
Lipika Singhal, Megha Sharma, Salony Verma, Ramanpreet Kaur, Xavier Basil Britto, Surbhi Malhotra Kumar, Pallab Ray, Vikas Gautam
ABSTRACT

With the increasing threat of multidrug-resistant organisms, such as Acinetobacter baumannii, the polymyxin class of drugs (colistin and polymyxin B) has become popular in clinical practice. A better understanding of antimicrobial susceptibility testing methods for colistin and polymyxin B is needed for optimal patient management. Forty-two carbapenem-resistant A. baumannii isolates were subjected to susceptibility testing for colistin and polymyxin B using the following methods: broth microdilution (BMD) (glass-coated plates [BMD-Gs] and polystyrene plates [BMD-Ps]), agar dilution (AD), E-test®, Vitek®, and disk diffusion. Using BMD as the gold standard, comparative analysis between different methods was carried out. With BMD-Gs as reference, reliability was high for BMD-Ps and moderate for AD and Vitek for both the drugs. Similar results were obtained when the BMD-P was used as reference, but drug-polystyrene interaction was observed. Different susceptibility testing methods for polymyxins show great variation in their results and BMD using glass-coated plates can be considered the best candidate for gold standard.

MATERIALS
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Supelco
Imipenem, Pharmaceutical Secondary Standard; Certified Reference Material
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EN300-15135-100MG100 mg
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CHF 363.50
EN300-15135-250MG250 mg
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CHF 500.00
EN300-15135-500MG500 mg
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EN300-15135-1G1 g
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EN300-15135-2.5G2.5 g
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EN300-15135-5G5 g
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EN300-15135-10G10 g
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CHF 3,995.70

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