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  • An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.

An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.

Nature communications (2018-04-29)
Haopeng Xiao, Weixuan Chen, Johanna M Smeekens, Ronghu Wu
ABSTRACT

Protein glycosylation is ubiquitous in biological systems and essential for cell survival. However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis. Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species. Here, we develop a strategy using dendrimer-conjugated benzoboroxole to enhance the glycopeptide enrichment. We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates. The enrichment is further improved by conjugating benzoboroxole to a dendrimer, which enables synergistic benzoboroxole-glycan interactions. This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides. Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Boc-Lys(Boc)-OSu, ≥97.0% (calc. based on dry substance, C/N)
Pricing and availability is not currently available. Contact Technical Service
Sigma-Aldrich
(3-Aminomethylphenyl)boronic acid hydrochloride, AldrichCPR
Pricing and availability is not currently available. Contact Technical Service