581425-U

Ascentis^® C8 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

• Synonym(s): C8 Reversed-Phase Chromatography Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® C8 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Agency: suitable for USP L7
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• extent of labeling: 15% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface area: 450 m²/g
• surface coverage: 4.0 μmol/m²
• impurities: <5 ppm metals
• matrix: fully porous particle; silica gel high purity, spherical
• matrix active group: C8 (octyl) phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.

Features and Benefits

• Superior retention for hydrophobic molecules
• Excellent peak shape
• Low bleed LC-MS separations

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany