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NA0310

Sigma-Aldrich

GenElute™ HP Plasmid Maxiprep Kit

greener alternative

sufficient for 25 purifications

Synonym(s):

GenElute™ HP Plasmid Maxiprep Kit, HP maxiprep kit, fast maxiprep kit, fast plasmid isolation, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

usage

sufficient for 25 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

greener alternative category

Aligned,

storage temp.

15-25°C

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General description

The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute® HP Plasmid Maxiprep Kit has been used in the isolation of plasmids.

Features and Benefits

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 1.2 mg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • Contains fewer plastic components than other high speed kits, reducing the amount of waste
  • No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

Gabitril is a registered trademark of Abbott Laboratories Corp.
GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

77.0 °F - closed cup

Flash Point(C)

25 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fraser Soares et al.
Blood, 137(16), 2171-2181 (2020-12-04)
Acute myeloid leukemia (AML) remains a devastating disease in need of new therapies to improve patient survival. Targeted adoptive T-cell therapies have achieved impressive clinical outcomes in some B-cell leukemias and lymphomas but not in AML. Double-negative T cells (DNTs)
Discovery of protein interactions using parallel analysis of translated ORFs (PLATO).
Larman HB
Nature Protocols, 9(1), 90-103 (2014)
Jaeho Yoon et al.
Biomedicines, 9(9) (2021-09-29)
Renal hypouricemia is a rare genetic disorder. Hypouricemia can present as renal stones or exercise-induced acute renal failure, but most cases are asymptomatic. Our previous study showed that two recessive variants of SLC22A12 (p.Trp258*, pArg90His) were identified in 90% of
Musaddeque Ahmed et al.
Nature communications, 12(1), 1781-1781 (2021-03-21)
Prostate cancer (PCa) risk-associated SNPs are enriched in noncoding cis-regulatory elements (rCREs), yet their modi operandi and clinical impact remain elusive. Here, we perform CRISPRi screens of 260 rCREs in PCa cell lines. We find that rCREs harboring high risk
Karina Yui Eto et al.
Antimicrobial agents and chemotherapy, 63(11) (2019-09-11)
Horizontal transfer of plasmids encoding antimicrobial resistance and virulence determinants has been instrumental in Staphylococcus aureus evolution, including the emergence of community-associated methicillin-resistant S. aureus (CA-MRSA). In the early 1990s, the first CA-MRSA strain isolated in Western Australia (WA), WA-5
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Protocols

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

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