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Key Documents

EC11303

Sigma-Aldrich

Escherichia coli

Strain B, lyophilized cells

Synonym(s):

E. coli

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.83

Quality Level

sterility

non-sterile (not processed or packaged aseptically)

form

lyophilized cells

suitability

not intended for starter culture

storage temp.

−20°C

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General description

Escherichia coli is a non-pathogenic, facultative anaerobic flora present in the intestine of human. It belongs to the genus, Escherichia, which consists of motile gram-negative bacilli.

Application

Escherichia coli has been used:
  • in the isolation of DNA from lyophilized cell
  • in the preparation of crude cell lysate for developing scalable purification method for recombinant protein A
  • as test proteome for comparative electrophoresis

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Accelerated recombinant protein purification process development: Automated, robotics-based integration of chromatographic purification and analysis
Londo T, et al.
Journal of Chromatography A, 798(1), 73-82 (1998)
Diarrheagenic Escherichia coli
Nataro JP and Kaper JB
Clinical Microbiology Reviews, 11(1), 142-201 (1998)
Severe atopic dermatitis is associated with a high burden of environmental Staphylococcus aureus
Leung AD, et al.
Clinical and Experimental Allergy, 38(5), 789-793 (2008)
Horizontal comparative fluorescence two-dimensional gel electrophoresis for improved spot coordinate detection
Hanneken M, et al.
Electrophoresis, 35(8), 1118-1121 (2014)
A D Leung et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 38(5), 789-793 (2008-03-18)
About 90% of patients with atopic dermatitis (AD) are colonized with Staphylococcus aureus. S. aureus worsens AD by secreting superantigens and structural molecules within the cell wall that induce skin inflammation. Therefore, S. aureus in the home may contribute to

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