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AC146

Sigma-Aldrich

Normal Chicken IgY Antibody, purified | AC146

1 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

purified antibody

clone

polyclonal

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

blocking: suitable (interfering antibodies)

isotype

IgY

shipped in

wet ice

target post-translational modification

unmodified

General description

Chickens transfer high quantities of IgY (IgG) into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One week′s eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. However, there are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian complement component C1 and it does not perform as a precipitating antibody using standard solutions.

Application

Gel Electrophoresis Analysis: 20 µg of reduced Chicken IgY from a representative lot was resolved by SDS-PAGE and visualized using total protein stain.

Note: To be used as a negative control for ChIP Ab+ MeCP2, Cat. No. 17-10491.

Note: For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction.
Research Category
Secondary & Control Antibodies
Research Sub Category
Isotype Control Antibodies

Quality

Evaluated by Western Blotting using non-reduced Chicken IgY.

Western Blotting Analysis: 0.01 µg of this non-reduced Chicken IgY was detected by a Rabbit anti-Chicken secondary antibody.

Target description

~180 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified
Purified chicken polyclonal in buffer containing PBS with 0.05% sodium azide.
T-gel

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nealia Cm House et al.
eLife, 8 (2019-12-06)
CAG/CTG trinuncleotide repeats are fragile sequences that when expanded form DNA secondary structures and cause human disease. We evaluated CAG/CTG repeat stability and repair outcomes in histone H2 mutants in S. cerevisiae. Although the two copies of H2A are nearly
Emile Rage et al.
Applied microbiology and biotechnology, 103(18), 7491-7504 (2019-07-25)
Infectious bursal disease virus (IBDV) is the cause of an economically important highly contagious disease of poultry, and vaccines are regarded as the most beneficial interventions for its prevention. In this study, plants were used to produce a recombinant chimeric
Tina M Kousted et al.
Heliyon, 3(3), e00267-e00267 (2017-04-04)
Hens have a tremendous capacity for producing polyclonal antibodies that can subsequently be isolated in high concentrations from their eggs. An approach for further maximizing their potential is to produce multiple antisera in the same individual through multiplexed (multiple simultaneous)
Piotr Michaluk et al.
eLife, 10 (2021-04-17)
Glutamate uptake by astroglial transporters confines excitatory transmission to the synaptic cleft. The efficiency of this mechanism depends on the transporter dynamics in the astrocyte membrane, which remains poorly understood. Here, we visualise the main glial glutamate transporter GLT1 by

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