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05-386

Sigma-Aldrich

Anti-Vinculin Antibody, clone V284

clone V284, Upstate®, from mouse

Synonym(s):

Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V284, monoclonal

species reactivity

human, rat, mouse, rabbit, chicken

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_003373

UniProt accession no.

P18206

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... VCL(7414)

Specificity

Vinculin

Immunogen

Human Vinculin

Application

Anti-Vinculin Antibody, clone V284 detects level of Vinculin & has been published & validated for use in IP, WB, IH.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

routinely evaluated by immunoblot on RIPA lysates of human A431 cells, lysates from chicken embryonic fibroblasts (CEF), or mouse 3T3/A31 fibroblasts

Target description

117kDa

Physical form

Format: Purified
PBS, pH 7.4, containing 0.05% sodium azide and 1mg/ml BSA
Protein A chromatography

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Paul G Leonard et al.
Nature chemical biology, 12(12), 1053-1058 (2016-10-25)
Despite being crucial for energy generation in most forms of life, few if any microbial antibiotics specifically inhibit glycolysis. To develop a specific inhibitor of the glycolytic enzyme enolase 2 (ENO2) for the treatment of cancers with deletion of ENO1
Unique morphology and focal adhesion development of valvular endothelial cells in static and fluid flow environments.
Butcher, JT; Penrod, AM; Garcia, AJ; Nerem, RM
Arteriosclerosis, Thrombosis, and Vascular Biology null
X Wu et al.
The Journal of biological chemistry, 276(32), 30285-30292 (2001-05-31)
The L-type calcium channel is the major calcium influx pathway in vascular smooth muscle and is regulated by integrin ligands, suggesting an important link between extracellular matrix and vascular tone regulation in tissue injury and remodeling. We examined the role
Temperature dependent specific heat capacity (Cp) of G-actin and talin or talin-vinculin bound to G-actin.
W H Goldmann et al.
Biochemical Society transactions, 20(3), 273S-273S (1992-08-01)
Millie Shah et al.
Molecular & cellular proteomics : MCP, 16(4 suppl 1), S244-S262 (2017-02-09)
Cellular responses to stimuli involve dynamic and localized changes in protein kinases and phosphatases. Here, we report a generalized functional assay for high-throughput profiling of multiple protein phosphatases with subcellular resolution and apply it to analyze coxsackievirus B3 (CVB3) infection
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