Common Markers and Small Molecule Inhibitors in Golgi Studies.

Common Markers and Small Molecule Inhibitors in Golgi Studies.

Methods in molecular biology (Clifton, N.J.) (2022-12-14)

Sarah Bui, Drew Stark, Jie Li, Jianchao Zhang, Yanzhuang Wang

ABSTRACT

In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology.

MATERIALS

Product Number

Brand

Product Description

L5288

Sigma-Aldrich

Latrunculin B from Latruncula magnifica, ≥80% (HPLC), solid

L6785

Sigma-Aldrich

Lactacystin, ≥90% (HPLC)

T7765

Sigma-Aldrich

Tunicamycin from Streptomyces sp.

C6628

Sigma-Aldrich

Chloroquine diphosphate salt, powder or crystals, 98.5-101.0% (EP)

K1385

Sigma-Aldrich

KN-93, ≥98% (HPLC)

M3694

Sigma-Aldrich

Anti-α1,2-Mannosidase IA antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

203290

Sigma-Aldrich

Bisindolylmaleimide I, A highly selective, cell-permeable, and reversible protein kinase C (PKC) inhibitor (IC₅₀ = 10 nM) that is structurally similar to staurosporine.

569397

Sigma-Aldrich

Staurosporine, Streptomyces sp., Staurosporine, CAS 62996-74-, is a cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinases (IC₅₀ = 7, 20, 1.3, 0.7, & 8.5 nM for PKA, CAMK, MLCK, PKC, & PKG, respectively).

565771

Sigma-Aldrich

γ-Secretase Inhibitor X, InSolution, ≥90%, 1 mM

654380

Sigma-Aldrich

Tunicamycin, Streptomyces lysosuperficus

SML0506

Sigma-Aldrich

LY-411575, ≥97% (HPLC)

11004646001

Roche

Antipain dihydrochloride, crystalline, suitable for cell culture, pkg of 10 mg

553210

Sigma-Aldrich

Rapamycin, ≥95% (HPLC), powder

579053

Sigma-Aldrich

TAPI-1, InSolution, ≥97%, inhibits MMPs and TACE

11004638001

Roche

Chymostatin

P0046

Sigma-Aldrich

Pyocyanin, from Pseudomonas aeruginosa, ≥98% (HPLC)

E3652

Sigma-Aldrich

Epoxomicin, ≥95% (HPLC), solid

A7250

Sigma-Aldrich

N-Acetyl-L-cysteine, Sigma Grade, ≥99% (TLC), powder

I2764

Sigma-Aldrich

ML-7, powder

E1383

Sigma-Aldrich

Etoposide, synthetic, 95.0-105.0%, powder

F0895

Sigma-Aldrich

FIBRONECTIN FROM HUMAN PLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture

A8456

Sigma-Aldrich

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)

474787

Sigma-Aldrich

MG-132, ≥95% by HPLC, Potent, reversible, and cell-permeable proteasome inhibitor (K_i = 4 nM).

196419

Millipore

BAPTA/AM, Membrane-permeable form of BAPTA.

565749

Sigma-Aldrich

β-Secretase Inhibitor II, The β-Secretase Inhibitor II, also referenced under CAS 263563-09-3, controls the biological activity of β-Secretase. This small molecule/inhibitor is primarily used for Neuroscience applications.

344827

Sigma-Aldrich

Fucosyltransferase Inhibitor, 2F-Peracetyl-Fucose, 2F-Peracetyl-Fucose is a cell-permeable fluorinated fucose derivative that acts as an inhibitor of fucosyltransferases following its uptake and metabolic transformation into a GDP-fucose mimetic.