04-1572-I
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
culture supernatant, clone 3E8, from rat
Synonym(s):
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
3E8, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
human (immunogen homology)
technique(s)
ChIP: suitable
ELISA: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer5)
Gene Information
human ... POLR2B(5431)
General description
RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-5′ CTD residue of RPB1.
Specificity
This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminus domain (CTD).
Immunogen
Epitope: Phosphorylated Ser5 at the C-terminus domain (CTD).
Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminal domain (CTD).
Application
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 is a highly specific rat monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting & ELISA.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blot in untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Target description
~220 kDa observed
Linkage
Replaces: 04-1572
Physical form
Rat monoclonal IgG2aκ with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Untreated and lambda phosphotase treated NIH/3T3 cell lysates
Untreated and lambda phosphotase treated NIH/3T3 cell lysates
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
In vivo live imaging of RNA polymerase II transcription factories in primary cells.
Ghamari, Alireza, et al.
Genes & Development, 27, 767-777 (2013)
Tanaz Sharifnia et al.
Nature medicine, 25(2), 292-300 (2019-01-22)
Chordoma is a primary bone cancer with no approved therapy1. The identification of therapeutic targets in this disease has been challenging due to the infrequent occurrence of clinically actionable somatic mutations in chordoma tumors2,3. Here we describe the discovery of
Joshua D Eaton et al.
eLife, 13 (2024-07-08)
RNA polymerase II (RNAPII) transcription initiates bidirectionally at many human protein-coding genes. Sense transcription usually dominates and leads to messenger RNA production, whereas antisense transcription rapidly terminates. The basis for this directionality is not fully understood. Here, we show that
Matthieu D Lavigne et al.
Nature communications, 8(1), 2076-2076 (2017-12-14)
Complex molecular responses preserve gene expression accuracy and genome integrity in the face of environmental perturbations. Here we report that, in response to UV irradiation, RNA polymerase II (RNAPII) molecules are dynamically and synchronously released from promoter-proximal regions into elongation
Dan Yu et al.
Nucleic acids research, 47(9), 4462-4475 (2019-03-14)
The general transcription factor P-TEFb, a master regulator of RNA polymerase (Pol) II elongation, phosphorylates the C-terminal domain (CTD) of Pol II and negative elongation factors to release Pol II from promoter-proximal pausing. We show here that P-TEFb surprisingly inhibits
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