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HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53825-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

standard

Product No.
Description
Pricing

47763

Cholecalciferol (D3)

analytical standard

47768

Ergocalciferol (D2)

analytical standard

CONDITIONS

column

Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)

mobile phase

acetonitrile

flow rate

0.15 mL/min

column temp.

35 °C

detector

UV, 290 nm

injection

10 μL

sample

10 mg/L each (vitamins D2 and D3) in methanol

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS