miR-210 expression is associated with methionine-induced differentiation of trout satellite cells.

miR-210 expression is associated with methionine-induced differentiation of trout satellite cells.

The Journal of experimental biology (2017-06-04)

Mary Latimer, Nathalie Sabin, Aurélie Le Cam, Iban Seiliez, Peggy Biga, Jean-Charles Gabillard

ABSTRACT

In fish, data on microRNAs (miRNAs) involved in myogenesis are scarce. In order to identify miRNAs involved in satellite cell differentiation, we used a methionine depletion/replenishment protocol to synchronize myogenic cell differentiation. Our results validated that methionine removal (72 h) from the medium strongly decreased

MATERIALS

Product Number

Brand

Product Description

N6635

Sigma-Aldrich

Nutrient Mixture F-10 Ham, With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

D7777

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

T9424

Sigma-Aldrich

TRI Reagent^®, For processing tissues, cells cultured in monolayer or cell pellets

D9785

SAFC

DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl_2,MgCl₂, MgSO_4,sodium bicarbonate, and phenol red, powder, suitable for cell culture