Method for screening of bacterial strains biosynthesizing specific conjugated linoleic acid isomers.

A simple and accurate method for screening of bacterial strains with the ability to convert free linoleic acid into specific conjugated linoleic acid (CLA) isomers has been developed by combining the ultraviolet spectral scan and capillary electrophoresis analysis. The ultraviolet spectral scan was carried out for preliminary screening of bacterial strains with the capacity to biosynthesize CLA, and the absorption peak at 228-235 nm was used for assessing the possible production of CLA by bacteria. The capillary electrophoresis analysis was used as the follow-up confirmation to definitively conclude CLA production and the composition of CLA isomers. Linoleic acid at the concentration of 25 μg/mL, which showed little inhibitory effect on the growth of bacteria, was used for initial screening of CLA-producing strains. The strains with the ability to produce specific CLA isomers can be selected quickly from a large number of bacteria by this high-throughput method.