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  • Heat Shock Proteins as Potential Indicators of Induced Stress in Nematodes.

Heat Shock Proteins as Potential Indicators of Induced Stress in Nematodes.

Methods in molecular biology (Clifton, N.J.) (2024-03-01)
Monica Celi, Mirella Vazzana, Barbara Manachini
ABSTRACT

Heat shock proteins (HSPs) in all animals studied to date constitute potential indicators of stress, under various environmental conditions. The goal of this chapter is to show, for the first time, the suitability of the approach based on evaluation of the expression levels of heat shock proteins, as good indicators of stress induced in nematodes by the cultivation of resistant plant varieties or by other potential stressors.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Heat Shock Protein 90 antibody produced in mouse, clone AC-16, ascites fluid
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment−Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-HSP27 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse, clone LK2, ascites fluid