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  • Absorbents as packing materials in on-line coupling of reversed phase liquid chromatography and gas chromatography via a programmed temperature vaporizer.

Absorbents as packing materials in on-line coupling of reversed phase liquid chromatography and gas chromatography via a programmed temperature vaporizer.

Journal of chromatography. A (2007-02-20)
Gema Flores, Maria Luisa Ruiz Del Castillo, Marta Herraiz
ABSTRACT

A method based on the use of absorbents as packing materials in the interface of the direct coupling between reversed phase liquid chromatography and gas chromatography (RPLC-GC) is proposed. To that end, a comparative study on different adsorbents and absorbents was carried out. Specifically, Tenax TA and Gaschrom were used as adsorbents while polydimethylsiloxane and poly(50% phenyl:50% methylsiloxane) were the absorbents tested. Some experimental variables involved in the solvent elimination were separately optimised for adsorbent and absorbent materials. Relative standard deviations (RSD) lower than 10% were achieved in all cases but the use of absorbents showed interesting advantages with respect to adsorbents, namely a simpler performance of the experimental work, which facilitates the sample preparation step and the subsequent gas chromatographic analysis to be performed.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Silicone oil AP 1000, viscosity ~1000 mPa.s,   neat(25 °C)
Sigma-Aldrich
Silicone oil AR 200, viscosity ~200 mPa.s,   neat(25 °C)
Sigma-Aldrich
Silicone oil AP 100, viscosity ~100 mPa.s,   neat(25 °C)
Sigma-Aldrich
Silicone oil AR 20, viscosity ~20 mPa.s,   neat(25 °C)