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Key Documents

SRP2120

Sigma-Aldrich

RNA Polymerase II Peptide

≥70% (SDS-PAGE), human recombinant, expressed in E. coli, GST tagged

Synonym(s):

POLRA, RPB1, RPO2, RPOL2, hRPB220

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

product name

RNA Polymerase II, C-terminal domain, GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

biological source

human

recombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

form

frozen liquid

mol wt

~68.1 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

color

clear colorless

NCBI accession no.

NM_000937

UniProt accession no.

P24928

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... POLR2A(5430)

Related Categories

Biochem/physiol Actions

The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA pol II in vivo: IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongation complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF. The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing.
The CTD works as a binding scaffold for nuclear factors through its phosphorylation. It is suggested to be also involved in chromatin structure modification, DNA damage/repair, protein degradation and synthesis, RNA degradation, snRNA (small nuclear RNA) modification, and snoRNP (small nucleolar ribonucleoprotein) biogenesis.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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RNA polymerase II C-terminal domain required for enhancer-driven transcription.
Gerber HP, et al.
Nature, 374, 660-662 (1995)
Phosphorylation and functions of the RNA polymerase II CTD.
Phatnani HP and Greenleaf AL
Genes & Development, 20, 2922-2936 (2006)
The role of multisite phosphorylation in the regulation of RNA polymerase II activity.
M E Dahmus
Progress in nucleic acid research and molecular biology, 48, 143-179 (1994-01-01)
T O'Brien et al.
Nature, 370(6484), 75-77 (1994-07-07)
The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II is essential in vivo, and is found in either an unphosphorylated (IIa) or hyperphosphorylated (IIo) form. The Drosophila uninduced hsp70 and hsp26 genes, and the constitutively expressed beta-1
H Lu et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(22), 10004-10008 (1991-11-15)
The two forms of RNA polymerase II that exist in vivo, phosphorylated (IIO) and nonphosphorylated (IIA), were purified to apparent homogeneity from HeLa cells. The nonphosphorylated form preferentially binds to the preinitiation complex. RNA polymerase II in the complex was
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