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10220647001

Roche

GTP-γ-S

Tetralithium salt

Synonym(s):

GTP-γ-S, GTP

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About This Item

UNSPSC Code:
12352204

description

C10H12N5O13P3SLi4

Quality Level

100

Assay

87% (HPLC)

form

solid

mol wt

Mr 539.2 (GTP-γ-S)
Mr 563.0 (GTP-γ-S-Li4)

composition

GDP, ≤12% HPLC
GMP, ≤1% HPLC
GTP, ≤2% HPLC

packaging

pkg of 10 mg

manufacturer/tradename

Roche

storage condition

protect from light

concentration

97%

technique(s)

activity assay: suitable

color

white

solubility

water: soluble

storage temp.

−20°C (−15°C to −25°C)

General description

Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors.

Application

GTP-γ-S has been used:
  • in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)
  • in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα)
  • to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants

Biochem/physiol Actions

Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically. It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),

Storage and Stability

Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.

Analysis Note

Absorption: Content is measured in aqueous solution by absorption at 254 nm.
Conditions:
  • approx. 10 mg/ml double-dist. water
  • dilution: 9.8 ml double-dist. water + 0.2 ml sample.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

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S Nawy et al.
Neuron, 7(4), 677-683 (1991-10-01)
Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement
Stephen R Sprang
Biopolymers, 105(8), 449-462 (2016-03-22)
This review addresses the regulatory consequences of the binding of GTP to the alpha subunits (Gα) of heterotrimeric G proteins, the reaction mechanism of GTP hydrolysis catalyzed by Gα and the means by which GTPase activating proteins (GAPs) stimulate the
Claudia Walliser et al.
The Journal of biological chemistry, 291(42), 22136-22148 (2016-08-21)
Mutations in the gene encoding phospholipase C-γ2 (PLCγ2) have been shown to be associated with resistance to targeted therapy of chronic lymphocytic leukemia (CLL) with the Bruton's tyrosine kinase inhibitor ibrutinib. The fact that two of these mutations, R665W and
Gilbert Di Paolo, Markus R. Wenk
Lipids, 3-18 (2012)
K Sasaki et al.
Nature, 325(6101), 259-262 (1987-01-15)
Recently, a GTP-binding protein sensitive to islet activating protein (IAP) has been suggested to be important in producing K+-currents when the muscarinic receptor of the atrial muscle is activated by acetylcholine (ACh). Here we confirm the blocking effects of IAP
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