HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18
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CONDITIONS
column
Discovery HS C18, 15 cm × 4.6 mm I.D., 3 μm particles (569252-U)
mobile phase
100% acetonitrile
flow rate
0.8 mL/min
column temp.
30 °C
detector
UV, 290 nm
injection
10 μL
sample
50 μg/mL each analyte
Description
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany