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  • In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.

In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.

Angewandte Chemie (International ed. in English) (2012-10-12)
Erkin Kuru, H Velocity Hughes, Pamela J Brown, Edward Hall, Srinivas Tekkam, Felipe Cava, Miguel A de Pedro, Yves V Brun, Michael S VanNieuwenhze
ABSTRACT

Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Benzofurazan, 97%
Sigma-Aldrich
FITC Labeled D-Alanine, Suitable for bacterial labeling
Sigma-Aldrich
Rhodamine labeled D-Lysine, Suitable for fluorescent microbial imaging
Sigma-Aldrich
Rhodamine Labeled D-galactosamine, Suitable for fluorescent microbial imaging
Sigma-Aldrich
FITC Labeled D-Lysine, Suitable for bacterial labeling
Sigma-Aldrich
Coumarin Labeled D-Lysine, Suitable for fluorescent microbial imaging