N3910
Neutralization Solution B
Synonym(s):
DNA neutralization solution, neutralization buffer DNA extraction
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About This Item
UNSPSC Code:
12161703
NACRES:
NA.25
Recommended Products
grade
for molecular biology
form
liquid
technique(s)
DNA amplification: suitable
DNA extraction: suitable
storage temp.
−20°C
General description
This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.
Application
Neutralization Solution B has been used in the modified Sigma Extract-N-Amp ™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp™ PCR Reaction Mix.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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