84100

Sucrose

suitable for microbiology, ACS reagent, ≥99.0%

• Synonym(s): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
• Empirical Formula (Hill Notation): C₁₂H₂₂O₁₁
• CAS Number: 57-50-1
• Molecular Weight: 342.30
• Beilstein: 90825
• EC Number: 200-334-9
• MDL number: MFCD00006626
• UNSPSC Code: 41106212
• PubChem Substance ID: 329769289
• NACRES: NA.85

Properties

• grade: ACS reagent
• Quality Level: 200
• Assay: ≥99.0% (HPLC); ≥99.0%
• form: powder
• optical activity: [α]20/D 66.3 to 66.8°, c = 26% in H₂O
• shelf life: limited shelf life, expiry date on the label
• packaging: pkg of 1 kg; pkg of 250 g; pkg of 5 kg
• storage condition: (Tightly closed)
• impurities: ≤0.0008 meq/g free acid; ≤0.005% water insoluble matter; ≤0.05% invert sugar; ≤5 mg/kg heavy metals (as Pb)
• ign. residue: ≤0.01% (as SO₄)
• loss: ≤0.03% loss on drying, 105 °C
• pH: 7.0 (20 °C, 100 g/L in water)
• mp: 185-187 °C (lit.)
• solubility: H₂O: 0.1 g/mL, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg; sulfate, sulfite (as SO₄^2-): ≤50 mg/kg
• cation traces: As: ≤0.1 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• application(s): microbiology
• SMILES string: OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
• InChI key: CZMRCDWAGMRECN-UGDNZRGBSA-N

Description

General description

Sucrose can be used as a carbon source in microbial growth media. It provides a source of energy and carbon for bacteria and supports their growth. It is used to prepare several types of media for the isolation and cultivation of various bacteria, fungi, and yeasts. Sucrose is commonly used in media for the identification and differentiation of various bacteria that grow on sucrose-containing media. Chromogenic agar media, such as MacConkey agar media with sucrose, are also commonly used in the identification of bacterial strains. Sucrose can also be used in specialized media for the isolation and culture of certain organisms, such as lactic acid bacteria, which are commonly used in the food industry. Additionally, sucrose can serve as a differential reagent in media formulations, such as TSI test (triple sugar iron test), which differentiates various Enterobacteriaceae bacteria based on their ability to ferment various types of sugars.

Application

Sucrose is commonly used as an ingredient in many microbial culture media for the growth and identification of bacteria, yeasts, and molds from various pharmaceutical, environmental, and food and beverage samples.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves