CBL194F
Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated
clone LP5K, Chemicon®, from mouse
Synonym(s):
Cytokeratin 7 Antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
saturated ammonium sulfate (SAS) precipitated
antibody product type
primary antibodies
clone
LP5K, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... KRT7(3855)
Specificity
The antibody reacts with cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.
FUSION PARTNER: Sp2/0
FUSION PARTNER: Sp2/0
Immunogen
Cytoskeletal proteins from cultured HeLa cells
Application
Detect Cytokeratin 7 using this Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated validated for use in IC, IH, IH(P), FC.
Immunohistochemistry: Suitable for staining frozen sections and paraffin wax embedded tissue. Protease treatment of paraffin embedded sections is required prior to antibody application. Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).
Immunocytochemistry: isolated EVT cells fixed with 0.5% PFA at 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).
Flow cytometry: 10μL of conjugate per test.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: isolated EVT cells fixed with 0.5% PFA at 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).
Flow cytometry: 10μL of conjugate per test.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Quality
Tested
Physical form
The antibody is ammonium sulphate precipitation followed by ion exchange chromatography. The conjugate is supplied in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin.
Storage and Stability
Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.
Other Notes
Please refer to lot specific datasheet.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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