HPLC Analysis of Column Test Mix on Discovery® BIO Wide Pore C18, Effect of HPLC Column I.D. on Sensitivity
CONDITIONS
column
Discovery BIO Wide Pore C18, 10 cm x various I.D., 3 μm particles
mobile phase
[A] water; [B] acetonitrile; (65:35, A:B)
flow rate
as indicated
column temp.
ambient
detector
UV, 254 nm
injection
as indicated
sample
acetophenone (0.1 μg), benzene (1 μg), toluene (1 μg)
Description
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany