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  • LINC00630 promotes cholangiocarcinoma cell proliferation, migration and invasion by mediating the miR-199a/FGF7 axis.

LINC00630 promotes cholangiocarcinoma cell proliferation, migration and invasion by mediating the miR-199a/FGF7 axis.

Journal of Cancer (2022-02-15)
Baiyin Zhong, Caixin Song, Qingfang He, Zhixi Chen, Qicheng Liao, Qiusheng Xiong, Shijie Wang, Yuansheng Xiao, Xing Xie, Yuankang Xie, Xiaonong Wang, Jianhong Zhang
ABSTRACT

Cholangiocarcinoma (CCA) is a type of cancer with a relatively low morbidity, but poor prognosis. Aberrant long non-coding RNA (lncRNA) expression has been observed in the pathological development of CCA. In the present study, lncRNA long intergenic non-protein coding RNA 630 (LINC00630) was found to be significantly upregulated in CCA tissues and cultured cells. LINC00630 expression was positively associated with histological differentiation, TNM stage and lymph node invasion. Short hairpin RNA (sh)-LINC00630 transfection could effectively decrease CCA cell proliferation, migration and invasion. Further investigations found that LINC00630 could interact with microRNA (miR)-199a, which specifically targeted fibroblast growth factor 7 (FGF7) for degradation. FGF7 overexpression restored the sh-LINC00630 transfection-induced decrease in CCA cell proliferation, migration and invasion. In conclusion, LINC00630 significantly promoted CCA cell proliferation, migration and invasion by upregulating FGF7 through miR-199a sponging.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-pan Ago Antibody, clone 2A8, clone 2A8, from mouse
Sigma-Aldrich
Anti-GAPDHS antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
IgG from sheep serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Sigma-Aldrich
Anti-FGF7 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Sheep-Anti-Rabbit IgG secondary Antibody, 2 mg/mL, Chemicon®