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  • An effective dual-factor modified 3D-printed PCL scaffold for bone defect repair.

An effective dual-factor modified 3D-printed PCL scaffold for bone defect repair.

Journal of biomedical materials research. Part B, Applied biomaterials (2020-01-07)
Yan Li, Qian Li, Hongming Li, Xiao Xu, Xiaoming Fu, Jijia Pan, Hui Wang, Jerry Ying Hsi Fuh, Yanjie Bai, Shicheng Wei
ABSTRACT

Numerous bioactive molecules produced in cells are involved in the process of bone formation. We consider that appropriate, simultaneous application of two types of bioactive molecules would accelerate the regeneration of tissues and organs. Therefore, we combined aspirin-loaded liposomes (Asp@Lipo) and bone forming peptide-1 (BFP-1) on three dimensional-printed polycaprolactone (PCL) scaffold and determined whether this system improved bone regeneration outcomes. in vitro experiments indicated that Asp@Lipo/BFP-1at a 3:7 ratio was the best option for enhancing the osteogenic efficiency of human mesenchymal stem cells (hMSCs). This was confirmed in an in vivo cranial defect animal model. In addition, RNA-Seq was applied for preliminarily exploration of the mechanism of action of this composite scaffold system, and the results suggested that it mainly improved bone regeneration via the PI3K/AKT signaling pathway. This approach will have potential for application in bone tissue engineering and regenerative medicine.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Edelfosine, ≥95% (HPLC)
Avanti
Egg Liss Rhod PE, Avanti Research - A Croda Brand 810146C
Avanti
Egg Liss Rhod PE, Avanti Research - A Croda Brand 810146P, powder