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  • In vivo gene modification elucidates subtype-specific functions of alpha(2)-adrenergic receptors.

In vivo gene modification elucidates subtype-specific functions of alpha(2)-adrenergic receptors.

The Journal of pharmacology and experimental therapeutics (2000-03-29)
J W Kable, L C Murrin, D B Bylund
ABSTRACT

Mice with altered alpha(2)-adrenergic receptor genes have become important tools in elucidating the subtype-specific functions of the three alpha(2)-adrenergic receptor subtypes because of the lack of sufficiently subtype-selective pharmacological agents. Mice with a deletion (knockout) of the alpha(2A)-, alpha(2B)-, or alpha(2C)-gene as well as a point mutation of the alpha(2A)-gene (alpha(2A)-D79N) and a 3-fold overexpression of the alpha(2C)-gene have been generated. Studies with these mice indicate that most of the classical functions mediated by the alpha(2)-adrenergic receptor, such as hypotension, sedation, analgesia, hypothermia, and anesthetic-sparing effect, are mediated primarily by the alpha(2A)-subtype. The alpha(2B)-subtype is the principal mediator of the hypertensive response to alpha(2)-agonists, appears to play a role in salt-induced hypertension, and may be important in developmental processes. The alpha(2C)-subtype appears to be involved in many central nervous system processes such as the startle reflex, stress response, and locomotion. Both the alpha(2A)- and alpha(2C)-subtypes are important in the presynaptic inhibition of norepinephrine release and appear to have distinct regulatory roles. The ability to study subtype-specific functions in different mouse strains by altering the same alpha(2)-adrenergic receptor in different ways strengthens the conclusions drawn from these studies. Although these genetic approaches have limitations, they have significantly increased our understanding of the functions of alpha(2)-adrenergic receptor subtypes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
ChemiSCREEN Human α2B Adrenergic Receptor Membrane Preparation, Human alpha2B GPCR membrane prepation for Radioligand binding Assays.
Sigma-Aldrich
ChemiSCREEN Human α2A Adrenergic Receptor Membrane Preparation, Human alpha2A GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.