V2258
Monoclonal Anti-Vimentin antibody produced in mouse
clone LN-6, ascites fluid
Synonym(s):
Anti-F5H288
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
LN-6, monoclonal
contains
15 mM sodium azide
species reactivity
pig, mouse, sheep, human, bovine, rabbit, feline, rat
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable using cultured cells
western blot: suitable
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)
General description
Vimentin belongs to the class of type III intermediate filament protein family. The gene is located on human chromosome 10p13. It is a cytoskeletal protein. Vimentin is mainly expressed in normal mesenchymal cells.
Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit.
Specificity
Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas.
Immunogen
human thymic nuclear extract
Application
Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) .
Monoclonal Anti-Vimentin antibody produced in mouse has been used in western blotting and immunolabeling.
Biochem/physiol Actions
Vimentin plays an important role in modulating cell growth. It plays a pivotal role in epithelial-mesenchymal transition. Vimentin acts as a potential target for anti-metastasis therapy for nasopharyngeal carcinoma (NPC). It regulates cellular architecture and tissue integrity. It is also involved in tumorigenesis and the metastatic spread of cancer. Vimentin provides stress resistance. It might be involved in neuritogenesis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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