The UPS FASTA file is accessible from the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/protein-mass-spectrometry/ups1-and-ups2-proteomic
UPS2
Proteomics Dynamic Range Standard Set
Protein Mass Spectrometry Calibration Standard
Synonym(s):
Dynamic Range Standards, Proteomics Standard Set
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biological source
human
Quality Level
form
ready-to-use solution
quality
Protein Mass Spectrometry Calibration Standard
concentration
10.6 μg/ampule protein
technique(s)
mass spectrometry (MS): suitable
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.
Application
Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins,[1] embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes.[2] It has also been used as a standard to spike HeLa cells for label-free quantification.[3]
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]
Kit Components Also Available Separately
Product No.
Description
SDS
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Regarding SKU# UPS2, can you please send me FASTA file of 48 proteins in UPS2 product? thanks you
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