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Key Documents

C2787

Sigma-Aldrich

Complement C7 from human serum

≥85% (SDS-PAGE), ≥150,000 C7H50 units/mg protein

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352205
NACRES:
NA.75

biological source

human serum

Quality Level

Assay

≥85% (SDS-PAGE)

form

solution

specific activity

≥150,000 C7H50 units/mg protein

concentration

1 mg/mL in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2

technique(s)

activity assay: suitable

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... C7(730)

Biochem/physiol Actions

The complement system is a complex cascade involving proteolytic cleavage of serum glycoproteins often activated by cell receptors. This cascade ultimately results in induction of the inflammatory response, phagocyte chemotaxis and opsonization, and cell lysis.

A complex of complements C5b, C6, C7, and C8 mediates the polymerization of up to eighteen C9 molecules into a tube-like membrane attack complex that is inserted into the plasma membrane of unwanted organisms, such as of Gram-negative bacteria and viral infected cells. This channel through the lipid bilayer results in lysis of the cell.

Human Complement C7 is a β-2-globulin, which is composed of a single polypeptide chain. The initial binding of C7 and C6 to C5b is thought to form a stable complex for subsequent binding of C8 and C9. The resulting activated complex is responsible for membrane attack and lysis.

Quality

Functionally active by a sensitive hemolytic assay.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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