C-12533
Human Aortic Smooth Muscle Cells (HAoSMC)
500,000 cryopreserved cells
Synonym(s):
Aortic Smooth Muscle Cells, Human Smooth Muscle Cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Recommended Products
biological source
human aorta (smooth muscles)
packaging
pkg of 500,000 cells
morphology
(smooth muscle)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
hypertension; cardiovascular diseases
shipped in
dry ice
storage temp.
−196°C
General description
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Cell Line Origin
Aorta
Application
Primary Human Aortic Smooth Muscle Cells (HAoSMC) are isolated from plaque-free regions of the human aorta and are provided in a cryopreserved format. HAoSMC stain positive for smooth muscle α-actin. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. The smooth muscle cells in the aortic wall subsequently contract it again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process. HAoSMC are suitable for studying the role of smooth muscle cells under normal or disease conditions in vitro.
Quality
Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subculture Routine
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Other Notes
Recommended Plating Density: 7500 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15
Recommended products
Recommended Primary Cell Culture Media:Link
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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